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- W2359802581 abstract "A serum-free culture system was used to study the in vitro culture of the preantral follicles from bovine ovaries. Follicles with diameters of 100-200 μm, dissected mechanically from ovarian cortical slices, were cultured individually in 96-well plates with 250 μL McCoy′s 5a medium supplemented with 20 mmol/L Hepes, 3 mmol/L L-glutamine, 0.1% BSA, 29 μg/L testosterone, 2.5 mg/L transferrin and 4 μg/L selenium. The follicles were incubated in a humidified atmosphere with 5% CO 2 at 37℃. The culture medium was changed every 6 days. The results showed that the follicles grew continuously maintaining their normal morphology. Antrum formation occurred after about 10 days of culture. The addition of insulin to the culture medium stimulated the growth of preantral follicles. However, similar effect was not shown for the addition of FSH." @default.
- W2359802581 created "2016-06-24" @default.
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- W2359802581 date "2002-01-01" @default.
- W2359802581 modified "2023-09-23" @default.
- W2359802581 title "In Vitro Culture of Preantral Folliles from Bovine Ovaries" @default.
- W2359802581 hasPublicationYear "2002" @default.
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