Matches in SemOpenAlex for { <https://semopenalex.org/work/W2359820553> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2359820553 abstract "Objective To explore the effects of biomimetic electrical stimulation on inducing differentiation of rat bone marrow mesenchymal stem cells(MSCs) into cardiomyocyte-like cells in isolated myocardium.Methods MSCs and cardiac myocytes from SD rats were isolated and cultured by modified method.MSCs were then co-cultured with cardiac myocytes(MSCs/ cardiomyocyte co-culture system).The co-culture system was divided into stimulated group and non-stimulated group.MSCs cells in stimulated group were given supra-threshold square biphasic pulses stimulation(5ms duration,0.5Hz,10V) lasting for 2h each day,while in non-stimulated group were routinely cultured.Each group was divided into three subgroups according to the incubation time of 3,5 and 7 days.The characteristic morphology of MSCs in every subgroup was observed under light microscope.The expression of cTnT in MSCs was detected by immunofluorescence.The rate of cTnT positive expression was also compared between two groups.Results The growth and frequency of myocardial wave of MSCs cells in stimulated group were better or higher than those in non-stimulated group.No expression of cTnT was detected in non-stimulated group at the 5th day of the experiment,while a few MSCs cells in the stimulated group expressed cTnT at the same time point.The cTnT expression was detected in the MSCs cells in the both groups on the 7th day.The rate of cTnT positive expression in stimulated group(20.98%±5.55%) was significantly higher than that in non-stimulated group(13.20%±3.98%,P0.05) at this time point.Conclusion Biomimetic electrical stimulation could promote the differentiation of rat bone marrow MSCs to cardiomyocyte induced by microenvironmental factors of cardiomyocyte in vitro,which with earlier exhibition of cardiomyocyte-like cells,and higher efficiency of differentiation rate." @default.
- W2359820553 created "2016-06-24" @default.
- W2359820553 creator A5083431227 @default.
- W2359820553 date "2009-01-01" @default.
- W2359820553 modified "2023-09-26" @default.
- W2359820553 title "Effects of biomimetic electrical stimulation on inducing differentiation of rat bone marrow mesenchymal stem cells into cardiomyocyte-like cells in isolated myocardium" @default.
- W2359820553 hasPublicationYear "2009" @default.
- W2359820553 type Work @default.
- W2359820553 sameAs 2359820553 @default.
- W2359820553 citedByCount "0" @default.
- W2359820553 crossrefType "journal-article" @default.
- W2359820553 hasAuthorship W2359820553A5083431227 @default.
- W2359820553 hasConcept C115725540 @default.
- W2359820553 hasConcept C126322002 @default.
- W2359820553 hasConcept C134018914 @default.
- W2359820553 hasConcept C159654299 @default.
- W2359820553 hasConcept C16685009 @default.
- W2359820553 hasConcept C185592680 @default.
- W2359820553 hasConcept C198826908 @default.
- W2359820553 hasConcept C202751555 @default.
- W2359820553 hasConcept C203014093 @default.
- W2359820553 hasConcept C207200792 @default.
- W2359820553 hasConcept C24998067 @default.
- W2359820553 hasConcept C2778236600 @default.
- W2359820553 hasConcept C2780007613 @default.
- W2359820553 hasConcept C28328180 @default.
- W2359820553 hasConcept C500558357 @default.
- W2359820553 hasConcept C55493867 @default.
- W2359820553 hasConcept C71924100 @default.
- W2359820553 hasConcept C86803240 @default.
- W2359820553 hasConcept C8880881 @default.
- W2359820553 hasConcept C95444343 @default.
- W2359820553 hasConceptScore W2359820553C115725540 @default.
- W2359820553 hasConceptScore W2359820553C126322002 @default.
- W2359820553 hasConceptScore W2359820553C134018914 @default.
- W2359820553 hasConceptScore W2359820553C159654299 @default.
- W2359820553 hasConceptScore W2359820553C16685009 @default.
- W2359820553 hasConceptScore W2359820553C185592680 @default.
- W2359820553 hasConceptScore W2359820553C198826908 @default.
- W2359820553 hasConceptScore W2359820553C202751555 @default.
- W2359820553 hasConceptScore W2359820553C203014093 @default.
- W2359820553 hasConceptScore W2359820553C207200792 @default.
- W2359820553 hasConceptScore W2359820553C24998067 @default.
- W2359820553 hasConceptScore W2359820553C2778236600 @default.
- W2359820553 hasConceptScore W2359820553C2780007613 @default.
- W2359820553 hasConceptScore W2359820553C28328180 @default.
- W2359820553 hasConceptScore W2359820553C500558357 @default.
- W2359820553 hasConceptScore W2359820553C55493867 @default.
- W2359820553 hasConceptScore W2359820553C71924100 @default.
- W2359820553 hasConceptScore W2359820553C86803240 @default.
- W2359820553 hasConceptScore W2359820553C8880881 @default.
- W2359820553 hasConceptScore W2359820553C95444343 @default.
- W2359820553 hasLocation W23598205531 @default.
- W2359820553 hasOpenAccess W2359820553 @default.
- W2359820553 hasPrimaryLocation W23598205531 @default.
- W2359820553 hasRelatedWork W2019337596 @default.
- W2359820553 hasRelatedWork W2063555845 @default.
- W2359820553 hasRelatedWork W2080832670 @default.
- W2359820553 hasRelatedWork W2122039545 @default.
- W2359820553 hasRelatedWork W2256567874 @default.
- W2359820553 hasRelatedWork W2352737845 @default.
- W2359820553 hasRelatedWork W2360941897 @default.
- W2359820553 hasRelatedWork W2367886038 @default.
- W2359820553 hasRelatedWork W2369222251 @default.
- W2359820553 hasRelatedWork W2370836592 @default.
- W2359820553 hasRelatedWork W2371723967 @default.
- W2359820553 hasRelatedWork W2375824355 @default.
- W2359820553 hasRelatedWork W2380032473 @default.
- W2359820553 hasRelatedWork W2380294516 @default.
- W2359820553 hasRelatedWork W2383974443 @default.
- W2359820553 hasRelatedWork W2388438499 @default.
- W2359820553 hasRelatedWork W2406459675 @default.
- W2359820553 hasRelatedWork W2901760820 @default.
- W2359820553 hasRelatedWork W3029277498 @default.
- W2359820553 hasRelatedWork W3032430155 @default.
- W2359820553 isParatext "false" @default.
- W2359820553 isRetracted "false" @default.
- W2359820553 magId "2359820553" @default.
- W2359820553 workType "article" @default.