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- W2359826114 abstract "Objective To construct efficient expression vector of HBsAg and B19-VP2 gene,and to detect the antigenicity of the expression products. Methods HBsAg gene and B19 VP2 gene were amplified by PCR.After being cloned with pGEM-T vector and sequencing,the HBsAg gene was inserted into the prokaryotic expression vector pQE30.After the recombinant pQE30-HBs had been formed,the VP2 gene was inserted into pQE30-HBs from pGEM-T-VP2 to construct pQE30-HBs-VP2.Then the pQE30-HBs-VP2 was transformed into(E.coli) BL21(DE3),and induced to express the fusion proteins by IPTG.Finally,the methods of SDS-PAGE and Western-blot were used to detect the level and the antigenicity of the fusion proteins. Results pQE30-HBs-VP2 recombinant vector could express efficiently in BL21.And the result of Western-blot indicated that the recombinant fusion protein had the better antigenicity,as it could be recognized by the antibody of anti-HBs and anti-VP2. Conclusion The pQE30-HBs-VP2 expression vector has been constructed successfully,its expression production has both the antigenic characterization of HBsAg and B19 VP2 protein.And the expression system of HBs and B19 VP2 is constructed successfully.It may provide some information for developing B19 and HBsAg recombinant combined vaccine,and also provide potential antigen for multi-diagnostic reagents." @default.
- W2359826114 created "2016-06-24" @default.
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- W2359826114 date "2005-01-01" @default.
- W2359826114 modified "2023-09-23" @default.
- W2359826114 title "Clone and Expression of HBV-HBsAg and Human Parvovirus B19-VP2" @default.
- W2359826114 hasPublicationYear "2005" @default.
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