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- W2359893853 abstract "Objective To observe the influence of exogenous nerve growth factor( NGF) on the expression of nuclear transcription factor-κB( NF-κB) mRNA of Schwann cells( SCs). Methods Schwann cells were isolated and cultured in vitro. The positive cells were counted by using S-100 immunohistochemical staining. The fourth generation of high-quality cells were randomly divided into the experimental group( NGF),blocking group( NGF + anti-NGFR) and control group( PBS). The total mRNA in each group were extracted and observed by ultraviolet spectrophotometer and 1.5% agarose gel electrophoresis,respectively,and the expression of NF-κB mRNA were detected by real-time fluorescence quantitative PCR. Results The living cell morphologies of SCs cultured in vitro were consistent with those observed under inverted microscope. S-100 immunohistochemical staining showed that the purity of SCs was 91%. Ultraviolet spectrophotometer observed that A260/A280 was 1. 8-2. 2,1. 5% agarose gel electrophoresis showed that there were 28 and 18 s clear bands,which confirmed the quality of extracted mRNA was good. Real time fluorescence quantitative PCR in detection of expression of NF-κB mRNA showed that when compared with the control group and blocking group,the expression of NF-κB mRNA in the experimental group was significantly increased( all P 0. 01). Conclusions Exogenous NGF can up-regulate the expression of NF-κB mRNA." @default.
- W2359893853 created "2016-06-24" @default.
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- W2359893853 date "2014-01-01" @default.
- W2359893853 modified "2023-09-23" @default.
- W2359893853 title "Influence of exogenous nerve growth factor on expression of NF-κB mRNA of Schwann cells" @default.
- W2359893853 hasPublicationYear "2014" @default.
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