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- W2359897210 abstract "Using Vero-E6 cell separation goat pox virus as coating antigen,a indirect competitive enzyme-linked immunosorbent assay for detection of goat pox antibody was developed and the reaction condition was optimized in indirect competitive ELISA.The optimal concentration of coating antigen in indirect competitive ELISA was 1 μg/ml,the optimal dilution of the rabbit anti-high was 1∶16 000,the optimal dilution of inspection of serum was 1∶400,and the cut-off value of 45.19% was set for the positive sera,and the cut-off value of 38.89% was set for the known negative sera.Out of 3 000 goat sera,83.33% were positive GPV by a indirect competitive ELISA,77.33%were by agar gel precipitation(AGP),the method of indirect competitive ELISA was more sensitive than the AGP.This studies reveal that the method has a high specificity,rapid and sensitive." @default.
- W2359897210 created "2016-06-24" @default.
- W2359897210 creator A5073561739 @default.
- W2359897210 date "2010-01-01" @default.
- W2359897210 modified "2023-09-24" @default.
- W2359897210 title "Establishment of Indirect Competitive Enzyme-linked Immunosorbent Assay of Goat Pox Antibody" @default.
- W2359897210 hasPublicationYear "2010" @default.
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