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- W2359905410 abstract "Objectives:To establish a method for separating extracellular proteins and membrane proteins in mycobacterium tuberculosis using two dimensional gel electrophoresis. Methods:Extracellular proteins and membrane proteins were extracted from H 37 Rv which were inoculated and cultured on Suton's medium for three weeks. The first dimensional electrophoresis was performed on immunobilized pH gradient rod gels (pH 3～10).Then proteins in the rod gels were separated using SDS PAGE gels. The silver stained gels were dried and scanned. The image manipulation was performed with Image Master 20 Elite 3.10. Results:Extracellular proteins and membrane proteins contained 907 and 681 protein spots, respectively, and most protein spots deriving from extracellular proteins and membrane proteins were in acidic range. In the basic range (pH more than 9.0),the number of protein spots belonging to extracellular proteins was only 10(1.1%),however, that of protein spots belonging to membrane proteins was 52(7.6%).In the acidic range (pH less than 4.0),the number of protein spots belonging to membrane proteins was only 15(2.2%),while that of protein spots belonging to extracellular proteins was 99( 10.9 %).The relative molecular weights of about 50% extracellular proteins and membrane proteins were less than 30 000. Conclusions:Two dimensional gel electrophoresis is useful to separate proteins in mycobacterium tuberculosis." @default.
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- W2359905410 date "2002-01-01" @default.
- W2359905410 modified "2023-09-28" @default.
- W2359905410 title "Separation of extracellular and membrane proteins in mycobacterium tuberculosis using two-dimensional gels electrophoresis" @default.
- W2359905410 hasPublicationYear "2002" @default.
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