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- W2359969797 abstract "Four induction protocols were designed for Lactobacillus lactis entering into viable but non-culturable(VBNC) state according to reverse adjustment protocol.Fluorescent images from VBNC L.lactis were observed with Live/Dead Bacterial Viability Kit.The VBNC bacterium resuscitation was conducted with Tween-80.Both protocol 2 in which L.lactis strains were cultured facultatively and anaerobicly with MRS from pH 2.0 to 3.0 at 4 ℃ and protocol 4 in which L.lactis strains were cultured facultatively and anaerobicly with MRS without cysteine from pH 2.0 to 3.0 at 4 ℃ could induce L.lactis strains to become VBNC state in a short time and maintain the state in a long time.Fluorescent observation showed that the cells of 3 strains were all atrophy,bending,connecting with each other,and altering original bacillus into streptobacillus.Connecting with each other was obviously increasing with the induction time,many streptobacillus showed red or green.VBNC L.lactis could be resuscitated to normal morphous and biochemical characteristics with Tween-80.The results indicated that L.lactis could become into VBNC state with different morphous." @default.
- W2359969797 created "2016-06-24" @default.
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- W2359969797 date "2008-01-01" @default.
- W2359969797 modified "2023-09-24" @default.
- W2359969797 title "Screening of conditions inducing Lactobacillus lactis to be viable but nonculturable state and fluorescence observation of VBNC L. lactis" @default.
- W2359969797 hasPublicationYear "2008" @default.
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