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- W2359979892 abstract "A sensitive and specific ELISA for SM2was established by anti-sulfamethazine monoclonal antibody. In the assay,sulfamethazine residue in sample was allowed to compete with the sulfamethazine-OVA conjugate immobilized on microtiter wells for binding to a limited amount of the anti-sulfamethazine monoclonal antibody, Horseradish peroxidase coupled to the goat IgG of anti-mouse IgG was selected as a tracer. Standard curves were constructed over 1 - 81 ng/ml of sulfamethazine,and the limit of detection of the ELISA was 1 ng/ml. Recoveries of sulfamethazine in spiked samples between 10, 60 and 200 ng/ml ranged from 64. 5% to 85. 5%. Precision of the assay as determined by interassay and intraassay variabilities was below 10%. Among 6 sulfonamide analogues tested for specificity .Sulfadimethoxine and sulfamer-azine showed significant cross-reaction in the assay. The assay is rapid,sensitive,and convenient to perform, and the test is readily adaptable to high-volume testing of sample in slaughterhouses." @default.
- W2359979892 created "2016-06-24" @default.
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- W2359979892 date "2003-01-01" @default.
- W2359979892 modified "2023-09-25" @default.
- W2359979892 title "Development of an enzyme linked immunosorbent assay kit for sulfamethazine in animal tissues" @default.
- W2359979892 hasPublicationYear "2003" @default.
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