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- W2359988940 abstract "[Objective] The paper aimed to study the biological function and group genetic variation situation of cattle Dmrt7 gene,which laid a foundation for the future study on the effects of this gene to the quality of cattle sperm.[Method] Cattle testis tissues as materials,2 pairs primers were designed and combined according to Dmrt7 gene sequence of mice published by GenBank,amplified through RT-PCR and sequence obtained from DNAMAN software and online tool were used for bioinformatics analysis;Meanwhile,11 tissues of cattle kidney,liver,testis,lung,spleen,rumen,uterus,small intestine,heart,ovary,muscle as materials,besides,2 pairs primers were designed for tissue expression profile analysis.Dmrt7 gene polymorphism were determined by PCR-SSCP technology and DNA sequence.[Result] cDNA fragment of 1 616 bp length(accession no is EF 534775),including ORF composed of 1 113 bases,this ORF coded 370 amino acids;and a C/G mutation was found on the 4th intron,and then group polymorphism were tested in the 227 local cattle varieties and abroad cattle varieties,the distribution range of G allele frequency was 0-0.413 8;the contents of gene heterozygosity,effective number of alleles and polymorphism information were 0-0.485 1,1.000 0-1.942 3,and 0-0.367 5.[Conclusion] The cDNA sequence of cattle Dmrt7 gene was cloned and tissue expression profile were analyzed,there were no difference between local cattle variety and abroad cattle variety in G allele." @default.
- W2359988940 created "2016-06-24" @default.
- W2359988940 creator A5071658109 @default.
- W2359988940 date "2009-01-01" @default.
- W2359988940 modified "2023-09-23" @default.
- W2359988940 title "Molecular Cloning and Genetic Variant of the Dmrt 7 gene in Cattle" @default.
- W2359988940 hasPublicationYear "2009" @default.
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