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- W2360024299 abstract "Objective To develop the minipool technology for detecti on of hepatitis C virus nucleic acid as a screening strategy of molecular epidem iology.Methods The use of fluorescence polymerase chain reaction determined the contents of hepatitis C virus cDNA in different minipool groups that minipools were formed in the stage of viral RNA extraction.The tests of individual sera were taken as the control group.Results It was found that the content of HCV cDNA for ext raction minipool group had no difference from that of the control group (P0. 05),but the content of HCV cDNA for reverse transcription group and extraction and reverse transcription group was lower than that of the control group (P0 .05)and weak positive sample missed the detection.Conclusion The minipool in the extraction stage was super ior to the minopool in the reverse tanscription stage for detection of HCV cDNA." @default.
- W2360024299 created "2016-06-24" @default.
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- W2360024299 date "2004-01-01" @default.
- W2360024299 modified "2023-09-23" @default.
- W2360024299 title "Study on different mnipool methods for detection of hepatitis C virus nucleic acid" @default.
- W2360024299 hasPublicationYear "2004" @default.
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