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- W2360062937 abstract "AIM To establish the correlation between the infection of human papillomavirus and occurrence of vulvar leukoplakia. METHODS A reverse hybridization blot probe assay was used.Six sequence specific oligonucleotides of HPVDNA (types 6, 11, 16, 18, 31 and 33) were used as specific probes and were fixed separately on a strip of nylon membrane, which could be used to hybridize with the PCR amplified target DNA sequence, so that we could detect and identify any one of the 6 types of HPV on the same strip. Paraffin sections of 58 patients with vulvar leukoplakia were tested for the presence of HPV by this assay and a general primer based PCR. RESULTS 22 out of 58 (37.9%) specimens tested were positive for HPV DNA by PCR, of which 7 were positive for HPV6 (31.8%), 5 for HPV11 (22.7%), 2 for HPV6/11 (mixed type) (9.1%), 10 for HPV16 (45.5%), 1 for HPV18 (4.5%), 0 for HPV31, 1 for HPV33 (4.5%). Statistical analysis revealed that there was marked correlation between the pathogeny of vulvar leukoplakia and HPVs ( P 0.01). CONCLUSION Infection of human papillomavirus may play a role in the occurrence of vulvar leukoplakia. We have identified both the benign and the malignant type HPVs in the vulvar leukoplakias." @default.
- W2360062937 created "2016-06-24" @default.
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- W2360062937 date "2001-01-01" @default.
- W2360062937 modified "2023-09-24" @default.
- W2360062937 title "Rapid detection and genotyping of human papillomavirus in patients with vulvar leukoplakia" @default.
- W2360062937 hasPublicationYear "2001" @default.
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