Matches in SemOpenAlex for { <https://semopenalex.org/work/W2360065881> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2360065881 abstract "【Objective】Establish the system of osteoclast-like cell(OLC) cultural in vitro and appraise the cultured cells were osteoclasts.【Methods】PBMCs were isolated by means of density gradient centrifugation from RA patients and were randomly assigned to 2 groups:group A(macrophage-colony stimulating factor,M-CSF,25 μg/L),group B(Receptor activator of NF-κB ligand,RANKL,5,10,20,25,50 μg/L,and 25 μg/L M-CSF).After cultured for 4,12,20 days,the cells on glass coverships were fixed and stained for tartrate resistant acid phosphatase,the bone slices were identified with toluidine blue stain.【Result】 The effect on the differentiation of OLC:RANKL induced a dose-dependent increase in TRAP positive OLC(P0.05).The effect on the activation of OLC:RANKL and M-CSF induced a dose-dependent increase in the number of bone resorptive lacuna on bone slices(P0.05),but we can not see bone resorptive lacuna on bone slices in group A.【Conclusion】RANKL and M-CSF can induce the differentiation and activation of osteoclast derived from PBMCs of patient with rheumatoid arthritis,this cultural method is convenient,potent and repretitive for OLC formation in vitro." @default.
- W2360065881 created "2016-06-24" @default.
- W2360065881 creator A5023506532 @default.
- W2360065881 date "2010-01-01" @default.
- W2360065881 modified "2023-09-28" @default.
- W2360065881 title "Culture of osteoclast-like cell obtained from peripheral blood mononuclear cells(PBMCs) of patients with rheumatoid arthritis in vitro" @default.
- W2360065881 hasPublicationYear "2010" @default.
- W2360065881 type Work @default.
- W2360065881 sameAs 2360065881 @default.
- W2360065881 citedByCount "0" @default.
- W2360065881 crossrefType "journal-article" @default.
- W2360065881 hasAuthorship W2360065881A5023506532 @default.
- W2360065881 hasConcept C126322002 @default.
- W2360065881 hasConcept C134018914 @default.
- W2360065881 hasConcept C137061746 @default.
- W2360065881 hasConcept C153911025 @default.
- W2360065881 hasConcept C170493617 @default.
- W2360065881 hasConcept C180675623 @default.
- W2360065881 hasConcept C181199279 @default.
- W2360065881 hasConcept C185592680 @default.
- W2360065881 hasConcept C199343813 @default.
- W2360065881 hasConcept C202751555 @default.
- W2360065881 hasConcept C203014093 @default.
- W2360065881 hasConcept C21547065 @default.
- W2360065881 hasConcept C2776033226 @default.
- W2360065881 hasConcept C2779244956 @default.
- W2360065881 hasConcept C2779428903 @default.
- W2360065881 hasConcept C2781282405 @default.
- W2360065881 hasConcept C50442881 @default.
- W2360065881 hasConcept C55493867 @default.
- W2360065881 hasConcept C71924100 @default.
- W2360065881 hasConcept C86803240 @default.
- W2360065881 hasConcept C88045685 @default.
- W2360065881 hasConceptScore W2360065881C126322002 @default.
- W2360065881 hasConceptScore W2360065881C134018914 @default.
- W2360065881 hasConceptScore W2360065881C137061746 @default.
- W2360065881 hasConceptScore W2360065881C153911025 @default.
- W2360065881 hasConceptScore W2360065881C170493617 @default.
- W2360065881 hasConceptScore W2360065881C180675623 @default.
- W2360065881 hasConceptScore W2360065881C181199279 @default.
- W2360065881 hasConceptScore W2360065881C185592680 @default.
- W2360065881 hasConceptScore W2360065881C199343813 @default.
- W2360065881 hasConceptScore W2360065881C202751555 @default.
- W2360065881 hasConceptScore W2360065881C203014093 @default.
- W2360065881 hasConceptScore W2360065881C21547065 @default.
- W2360065881 hasConceptScore W2360065881C2776033226 @default.
- W2360065881 hasConceptScore W2360065881C2779244956 @default.
- W2360065881 hasConceptScore W2360065881C2779428903 @default.
- W2360065881 hasConceptScore W2360065881C2781282405 @default.
- W2360065881 hasConceptScore W2360065881C50442881 @default.
- W2360065881 hasConceptScore W2360065881C55493867 @default.
- W2360065881 hasConceptScore W2360065881C71924100 @default.
- W2360065881 hasConceptScore W2360065881C86803240 @default.
- W2360065881 hasConceptScore W2360065881C88045685 @default.
- W2360065881 hasLocation W23600658811 @default.
- W2360065881 hasOpenAccess W2360065881 @default.
- W2360065881 hasPrimaryLocation W23600658811 @default.
- W2360065881 hasRelatedWork W2004129299 @default.
- W2360065881 hasRelatedWork W2029276167 @default.
- W2360065881 hasRelatedWork W2050345294 @default.
- W2360065881 hasRelatedWork W2348970111 @default.
- W2360065881 hasRelatedWork W2350326590 @default.
- W2360065881 hasRelatedWork W2353255029 @default.
- W2360065881 hasRelatedWork W2356680727 @default.
- W2360065881 hasRelatedWork W2360125695 @default.
- W2360065881 hasRelatedWork W2360259916 @default.
- W2360065881 hasRelatedWork W2377295054 @default.
- W2360065881 hasRelatedWork W2378761183 @default.
- W2360065881 hasRelatedWork W2383970599 @default.
- W2360065881 hasRelatedWork W2384143878 @default.
- W2360065881 hasRelatedWork W2390754171 @default.
- W2360065881 hasRelatedWork W2954136648 @default.
- W2360065881 hasRelatedWork W2978986095 @default.
- W2360065881 hasRelatedWork W3013864573 @default.
- W2360065881 hasRelatedWork W3028653243 @default.
- W2360065881 hasRelatedWork W3147152868 @default.
- W2360065881 hasRelatedWork W3149339154 @default.
- W2360065881 isParatext "false" @default.
- W2360065881 isRetracted "false" @default.
- W2360065881 magId "2360065881" @default.
- W2360065881 workType "article" @default.