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- W2360096721 abstract "Immature inflorescence segments of distant hybrids F 1 from Roegneria ciliaris var.japonensis×T.aestivum cv.J-11, R.kamoji×Hordeum vulgare, T.aestivum cv. Chinese Spring×Psathyrostachys huashanica as well as between Triticum durum Dasypyrum villosum amphidiploid and Psathyrostachys huashanica, were cultured on N 6 medium supplemented with colchicine of different concentration such as 0 0, 50 0, 100 0, 150 0, 200 0, 250 0 and 300 0mg/L besides 2,4-D, IAA, KT, CH and Inositol. The dual effects of colchicine in the culture were observed. On the one hand, colchicine stimulated the callus formation. For one thing, the speeds of forming callus on all treat medium were higher than that of the control (The colchicine is 0 0mg/L). For example, the two combinations such as R.ciliaris var. japonensis×T.aestivum cv.J-11 and T.aestivum cv.C.S×P.huashanica, 50% of the immature inflorescence segments formed calli only within seven days after cultured on the media containing colchicine, but on the conchicine free medium, less than 50% of the explants produced callus after 30 days inoculated. For another, the average callusing frequencies on the media containing colchicine were significant high compared with that of the control. For instance, the mean dedifferentiation frequency on the medium supplemented with 100 0 mg/L colchicine was 66 7%, which increased 66 8% against the control (40 0%), and the result by t test showed P was 0 01. On the other hand, colchicine rendered the yang inflorescence segments browning and even died. And the analysis through t test indicated the average browning frequencies from the four combinations were outstanding higher than that of the control." @default.
- W2360096721 created "2016-06-24" @default.
- W2360096721 creator A5055550617 @default.
- W2360096721 date "1999-01-01" @default.
- W2360096721 modified "2023-09-25" @default.
- W2360096721 title "Dual Effects of Colchicine in Immature Inflorescence Dedifferentiation of Distant Hybrids F_1 from Triticeae" @default.
- W2360096721 hasPublicationYear "1999" @default.
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