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- W2360097209 abstract "To reveal the expression of exogenous genes BtCry1Ac and NTHK1 of plant transformation vector p096899 harboring multi-genes in transgenic plants,the wild tobacco was transformed by Agrobacterium-mediated leaf disc transformation method.Ten strains of transgenic tobacco were initially screened out by kanamycin.Then,identified by PCR,the target genes were integrated into tobacco genome.Expression of target genes in transcription and protein levels were analyzed by real-time PCR and ELISA,and the strains of transgenic tobacco were also tested for insect-resistance and salt-resistance.The results showed that the target genes expressed in the transcription and protein levels,respectively,and there existed an expression amount difference among the strains.The toxic protein amount of the 2th strain was the highest,which accounted for 0.028 5% of the leaf total soluble proteins.Result of feeding insect experiment illustrated that toxic effect exerted by some of transgenic tobacco strains on Spodoptera litura was obviously stronger than that of untransformed strains(CK).The 2th strain was most insecttolerant,and the mortality rate of tested larvae reached 75.56% on the 6th day,which was consistent with ELISA test results.Under salt stress,the growth state of the transgenic tobacco leaves was better than CK.These results prove that introduction of BtCry1Ac and NTHK1 genes into tobacco could improve the insect-resistance and salt-resistance." @default.
- W2360097209 created "2016-06-24" @default.
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- W2360097209 date "2014-01-01" @default.
- W2360097209 modified "2023-09-30" @default.
- W2360097209 title "Tobacco Transformation with Bivalent Gene BtCry1Ac and NTHK1 Mediated by Agrobacterium" @default.
- W2360097209 hasPublicationYear "2014" @default.
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