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- W2360097497 abstract "Objective To compare the analgesic effect of extracts of Fructus Evodiae, and to determine the active ingredient contents of Fructus Evodiae. Methods Analgesic effects of Fructus Evodiae extracts on acetic acid writhing method and pain mice model induced by formalin was observed. HPLC was adopted for the determination of active ingredient contents of Fructus Evodiae. The separation was performed on the Phenomenex C18(4.6 mm×250 mm,4μm) column with methanol- water as mobile phase in gradient dilution. The flow rate was set at 1.0 mL·min-1,and the ultraviolet detector was operated at 230 nm. Results All of the three different extracts of Fructus Evodiae can inhibit mice writhing reaction and shorten the foot licking time of mice in the second phase in formalin- induced pain mice model,and the effect of ethanol extract was more obvious than the others. The linear ranges of evodin,evodiamine and rutaecarpine were 80 ~ 500 μg·mL-1(r=0.9996),0 ~ 80 μg·mL-1(r=0.9998)and 0 ~ 80 μg·mL-1(r=0.9997),respectively. The extract rates of evodin, evodiamine and rutaecarpine were 0.747 %, 0.009 % and 0.004 %,respectivelyin the water extract ofFructus Evodiae,were 1.217 %,0.200 % and 0.129 % in the methanol extract,and became 1.220 %,0.201 % and 0.154 % in the ethanol extract. Conclusion The analgesic effect of ethanol extract of Fructus Evodiae is superior to that of methanol extract and water extract,and ethanol extract has higher content of active ingredients." @default.
- W2360097497 created "2016-06-24" @default.
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- W2360097497 date "2014-01-01" @default.
- W2360097497 modified "2023-09-23" @default.
- W2360097497 title "Comparison of Analgesic Effect and Active Ingredient Content of Fructus Evodiae Extracts" @default.
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