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- W2360127878 abstract "A strategy for purification full length human peroxisome proliferator activated receptor-γ(PPARγ)was developed by immobilized metal-ion affinity chromatography.The full length human PPARγRecombinant Protein was expressed by E.coli BL_(21)(DE3);and purification was carried out by immobilized metal-ion affinity chromatography.The conditions;50mmol/L imidazole in washing buffer and 200mmol/L imidazole in elution buffer with 8mol/L Urea,that was optimized by examining imidazole concentration.95%purity of the full length PPAR7 was obtained by one-step purification with IMAC.After dialysis refolded,the activity was tested with radio-ligand receptor binding assay and the K_d was 106±6nmol/L.These results demonstrate that the strategy here can be used to produce biologically activie full length human PPAR7 recombinant protein for the investigation of structure and function." @default.
- W2360127878 created "2016-06-24" @default.
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- W2360127878 date "2013-01-01" @default.
- W2360127878 modified "2023-09-25" @default.
- W2360127878 title "Purification of Full Length Human PPARγRecombinant Protein by Immobilized Metal-ion Affinity Chromatography" @default.
- W2360127878 hasPublicationYear "2013" @default.
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