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- W2360162005 abstract "Objective To establish a real-time fluorescence quantitative polymerase chain reaction(RTFQ-PCR)method for detection of human receptor for advanced glycation end products(RAGE)mRNA and investigate RAGE expression in esophageal cancer tissue.Methods The specific primers were designed by the fifth and sixth extron sequence of RAGE according to GenBank database.Using SYBR Green I,a real-time quantitative PCR method was established,and its linearity,specificity,sensitivity and reproducibility were evaluated.Based on the standard curve created by plasmid DNA,the expression levels of RAGE in esophageal cancer and paired non-cancer tissue samples were determined and the results were presented as the ratio of RAGE mRNA to 18S rRNA.Results The linear range was 5.0x10(3)to 5.0x10(9)copies/ml.The coefficient correlation was-0.98.The intra-assay coefficient variation was 0.63% to-8.71%.The inter-assay coefficient variation was 1.52% to 12.38%.The relative expression levels of RAGE in esophageal cancer and paired non-cancer tissues were 0.638±0.068 and 0.334±0.329 respectively when 18S rRNA was used as the reference(P0.005).Analysis results indicated RAGE expression was up-regulated in the tissues of esophageal cancer.Conclusions RTFQ-PCR method for RAGE mRNA determination is sensitive,specific and reproductive.RAGE expression is associated with occurrence and progression of esophageal cancer." @default.
- W2360162005 created "2016-06-24" @default.
- W2360162005 creator A5057453932 @default.
- W2360162005 date "2008-01-01" @default.
- W2360162005 modified "2023-09-23" @default.
- W2360162005 title "Establishment of a real-time quantitative polymerase chain reaction for detection of human receptor for advanced glycation end products (RAGE) mRNA and its preliminary application" @default.
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