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- W2360167802 abstract "OBJECTIVE The aim of the study was to setup and optimize the explant culture system of olfactory receptor neurons(ORNs).METHODS Rat tail collagen was used as the culture substratum.The olfactory epithelium(OE)from the septum and ethmoid turbinates of newborn mice was harvested,plated and cultured.The morphology and growth pattern of cultured cells including ORNs were studied under inverted microscope and scanning electron microscope.The phenotype and maturity of the cultured cells were determined by means of Immunocytochemistry.RESULTS After optimization of the culture condition,fully developed ORNs were achieved.The OE explant culture was a heterogeneous culture system,which consisted of basal cells,olfactory ensheathing cells(OECs),fusiform cells,and fibroblasts besides ORNs.The basal cells and fusiform cells were characterized as precursors of ORNs.The ORNs maintained stably beyond 9 days in vitro.CONCLUSION Using explant culture,which devoid the potential damage of any enzyme,fully developed ORNs could be stably achieved." @default.
- W2360167802 created "2016-06-24" @default.
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- W2360167802 date "2010-01-01" @default.
- W2360167802 modified "2023-09-26" @default.
- W2360167802 title "Explant culture of olfactory epithelium" @default.
- W2360167802 hasPublicationYear "2010" @default.
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