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- W2360179504 abstract "The interaction of 5'-monophosphate sodium salt(AMPNa)and human serum albumin(HSA)can form a kind of complex substances with no fluorescence.This can lead to change of the fluorescence of HSA.There is a linear relation between the intensity of synchronous fluorescence and the concentration of HSA.Then we establish a new method for the determination of proteins using the AMP-Na as probe by synchronous fluorescence technique.In this experiment,we investigate some factors includeΔλ,reaction medium and temperature on the synchronous fluorescence intensity.Under the optimum experimental conditions,the synchronous fluorescence intensities of AMPNa was in good proportion to the concentration of HSA in the range of 1.43~244.8μg·mL-1.The detection limit is 0.319μg·mL-1 by determination of 11blank solution.Determination of protein in serum,urine and saliva samples under the optimum conditions,the recovery is in the range 95.06%~104.89%." @default.
- W2360179504 created "2016-06-24" @default.
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- W2360179504 date "2014-01-01" @default.
- W2360179504 modified "2023-09-26" @default.
- W2360179504 title "Determination of Proteins Content in Biology Samples with Adenosine 5'-monophosphate sodium salt as Probe by Synchronous Fluorescence Technique" @default.
- W2360179504 hasPublicationYear "2014" @default.
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