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- W2360247002 abstract "Purpose To study the effect of HCMV on expression of cathepsin D in duct epithelial cells of salivary gland.Methods The expression of cathepsin D was detected by immunohistochemical staining in tissues embedded in paraffin of parotid cytomegalic inclusion disease (PCID).Effects of HCMV infection on cathepsin D expression of duct epithelial cell cultures of human salivary gland (HSG) in vitro were investigated by Western blotting.Semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to evaluate the transcription levels of cathepsin D of HSG infected by HCMV.Results Cathepsin D was positively expressed in inclusion-bearing cytomegaly of PCID tissues by immunohistochemistry.Both procathepsin D and its active heavy-chain form were up-regulated gradually but kept below the normal level while active single-chain form were up-regulated quickly and came to 1.91 times of normal level at 96 h post-infection.The mRNA levels were paralleled reversely with protein levels of cathepsin D of HSG.Conclusions Expression of active single-chain form of cathepsin D is induced to up-regulate in duct epithelial cells of salivary gland by HCMV." @default.
- W2360247002 created "2016-06-24" @default.
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- W2360247002 date "2010-01-01" @default.
- W2360247002 modified "2023-09-24" @default.
- W2360247002 title "Effect of HCMV on expression of cathepsin D in duct epithelial cells of salivary gland" @default.
- W2360247002 hasPublicationYear "2010" @default.
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