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- W2360289436 abstract "Protein interacting with Cα kinase 1(PICK1),an evolutionaryly conserved protein widely present in creatures from C.elegans to human being,is a peripheral membrane protein appeared in the cytoplasm and plays an important role in the process of protein translocation,and the intracellular signal transduction.PICK1 and its truncated fragments(N-PDZ and BAR-C) were expressed in E.coli BL21(DE3) and purified by affinity chromatography and gel filtration.PICK1 was in the form of dimer in solution by the analysis of non-denaturing PAGE and molecular exclusion chromatography.The binding properties of PICK1 with Ca~(2+) and Mg~(2+) was examined by fluorescence spectra.Ca~(2+) and Mg~(2+) have the similar affinity with PICK1 at 2∶1 stoichiometry in 0.02 mol·L~(-1) Hepes,pH 7.4 at 25 ℃.The conditional binding constants of complex PICK1-Ca~(2+) and PICK1-Mg~(2+) are K_(a1)=(2.34±0.20)×10~6 L·mol~(-1),K_(a2)=(7.75±0.62)×10~5 L·mol~(-1) and K_a=(5.00±0.40)×10~6 L·mol~(-1),respectively.Moreover,Ca~(2+) can bind to both N-PDZ and BAR-C regions of PICK1,while Mg~(2+) to N-PDZ region only.Compared to Mg~(2+),Ca~(2+) significantly enhances the lipid binding of PICK1 according to the fluorescence titration assays." @default.
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- W2360289436 date "2010-11-26" @default.
- W2360289436 modified "2023-09-26" @default.
- W2360289436 title "Oligomeric Properties of Recombinant PICK1 and Its Binding Capacity with Ca~(2+) and Mg~(2+)" @default.
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