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- W2360293183 abstract "Objective:To culture neural stem cell (NSC) in vitro,observe its biological property and provide basis for further study on differentiation.Methods:NSC of rat fetus were isolated and cultured.Single-cell clone culture was used to confirm the character of self-renewing.Ability of multi-directional differentiation and specific antigen, nestin,were detected by immunocytochemistry (ICC).BrdU was used to show cell stage in division.Effect of combination of EGF,bFGF on proliferation of rat NSC were evaluated.Results:The cultured NSC showed multipotential of differentiation into neurons and glial cells,as well as the ability of self-renewing.Cells expressed nestin.The population of the NSC expanded quickly and the total number of the cell increased 2.45×10~4 folds after 15 times' passage.The property of NSC was stable after long culture and preservation.Combination of EGF and bFGF stimulated cell proliferation than used singly.Conclusion:The cultured NSC express nestin, have the ability of self-renewing and multi-directional differentiation.Combination of EGF and bFGF could promote the proliferation of rat NSC." @default.
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- W2360293183 date "2004-01-01" @default.
- W2360293183 modified "2023-10-16" @default.
- W2360293183 title "Isolation,expansion and identification of fetal rat neural stem cell" @default.
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