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- W2360301885 abstract "【Objective】The aim is to characterize the OPB-08900 sequence and to identify the genetic distance between RAPD marker OPB-08900 and precocious gene of walnut(Juglans regia L.).【Method】The template DNA extracted from precocious maternal parent Luyuan,later mature paternal parent Lufeng and their 154 F1 hybrid plants was screened with OPB-08 primer(5'-GTCCACACGG-3').The genetic distance between OPB-08900 and the precocious walnut gene was calculated using Haldane function.DNA fragments of OPB-08900 derived from precocious F1 hybrid No.2 were retrieved from the agarose gel.After ligated into pMD18-T Vector,the DNA was transformed into E.coli JM109 and the positive clones were screened.The restriction sites and homologue of the sequence OPB-08958 were analyzed respectively by DNAMAN and BLAST in nr database of GenBank.【Result】 The RAPD-PCR results showed that specific fragment OPB-08900 was presented in precocious maternal parent Luyuan and 69 precocious F1 hybrid plants,but it was absent in later mature paternal parent Lufeng and 82 later mature F1 hybrid plants.The RAPD marker OPB-08900 was co-segregating with precocious trait of walnut and the genetic distance between OPB-08900 and precocious gene was 1.99 cM.The sequencing results showed that the RAPD marker OPB-08900 was 958 bp,so RAPD marker OPB-08900 should be renamed OPB-08958 with a GenBank accessioned number of DQ673614.There were 97 restriction sites in the sequence of OPB-08958,which could be digested by 36 restriction enzymes.The sequence of OPB-08958 was not homologous with any accessioned plants in GenBank.【Conclusion】The precocious trait of walnut might be controlled by more than 2 genes.RAPD marker OPB-08958 might link to one of the precocious genes and be unique to walnut genome." @default.
- W2360301885 created "2016-06-24" @default.
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- W2360301885 date "2007-01-01" @default.
- W2360301885 modified "2023-09-25" @default.
- W2360301885 title "RAPD Marker Linked to Precocious Gene of Walnut (Juglans regia L.) and Its Sequence Analysis" @default.
- W2360301885 hasPublicationYear "2007" @default.
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