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- W2360330526 abstract "Cinnamomum camphora's genomic DNA from young buds and dried buds with silica gel was extracted by the modified CTAB method.The results showed that polyphenolics were effectively removed from materials by blendering 3% PVP to the ground materials under liquid nitrogen in mortar and acceding concentration of 2% β-mercaptoethanol in extracting buffer;the polysaccharides were effectively removed by adding 1/20 volume 5 mol/L sodium chloride solution and absolute ethanol to the blundering aqueous phase after successively extracting lysis solution twice with 24∶1 chloroform∶isoamyl alcohol;the high and purer DNA samples were obtained.Though the average yield(373 ug/g) and purity(A_(260)/A_(280) between 1.54~1.92) of DNA extracted from dried buds were fewer than the DNA extracted from young buds,but the bands of RAPD-PCR amplified DNA were all clear,stable,bright and complete." @default.
- W2360330526 created "2016-06-24" @default.
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- W2360330526 date "2006-01-01" @default.
- W2360330526 modified "2023-09-26" @default.
- W2360330526 title "A Study on Methods for Extraction of DNA from Cinnamomum camphora Dry Leaves" @default.
- W2360330526 hasPublicationYear "2006" @default.
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