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- W2360372009 abstract "OBJECTIVE:To study the effect of supernatant fluid of lung carcinoma cells on the function and activity of endothelial progenitor cells.METHODS:The mononuclear cells were isolated from rat bone marrow by the ficoll density gradient centrifugation and cultured for 24 h.Then the suspending cells were cultured in the culture flask.After 5 days cultured,EPCs were identified as adherent cells double positive stained for AC133 and vWF under a laser confocal immunofluence microscope.Then the attched cells were treated with supernatant(volume fraction 0,10%,20% and 40%)for various time points(6,12,24 and 48 h).EPCs proliferation,migration and tubule formation capacity were assayed by the MTT assay,transwell chamber assay and matrigel tubule assay.RESULTS:The supernatant fluid of lung carcinoma cells promotes EPCs proliferative,migratory and in vitro tubule formation capacity(P0.05),which was a time and dose dependent manner.After 24 h affected,the OD490 value of EPCs proliferative in control was 0.332±0.051,that of volume fraction 10%,20% and 40% was 0.411±0.018(P0.05),0.446±0.036(P0.01)and 0.467±0.018(P0.01),respectively;the cell value of EPCs migratory in control was 37.0±3.937,that of volume fraction 10%,20% and 40% was 42.2±3.114(P0.05),44.8±3.564(P0.05)and 46.2±4.025(P0.01),respectively;the number of EPCs tubule formation in control was 7.0±0.71,that of volume fraction 10%,20% and 40% was 8.4±1.14(P0.05),9.2±1.30(P0.05)and 10.2±1.30(P0.01),respectively.CONCLUSIONS:In vitro the supernatant fluid of lung carcinoma cells can advance EPCs proliferative,migratory and tubule formation capacity with a time and dose dependent manner,which suggestes in vivo tumor cells may secrete some active material to enhance EPCs activity and facilitate vasculogenesis in tumor." @default.
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- W2360372009 date "2009-01-01" @default.
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- W2360372009 title "Effect of supernatant fluid of lung carcinoma cells on function and activity of endothelial progenitor cells" @default.
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