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- W2360415618 abstract "Objective To reconstruct shuttle plasmid pJEM11 into Mycobacterium shuttle expression plasmid(pJHSP70)with Mycobacterium tuberculosis HSP70 promoter and identify the function of pJHSP70.Methods The HSP70 promoter was amplified from the BCG DNA by PCR and cloned into pMD18-T vector.After identified by enzyme digestion analysis and sequencing,HSP70 promoter was subcloned into pJEM11 that was digested with Apa Ⅰ and SnaB Ⅰ,to form the plasmid pJHSP70.After pJHSP70 was amplified by PCR and identified with enzyme digestion analysis,Helicobacter pylori UreB(Hp UreB)gene was cloned into pJHSP70.The expression of Hp UreB protein in the Mycobacteria smegmatis mc2 155 was detected by SDS-PAGE.Results The fragment of 160 bp was amplified from the BCG DNA by PCR.Sequencing and homologous analysis showed that the homology between the cloned HSP70 gene and related genes in GenBank was 100%.SDS-PAGE analysis showed that Hp UreB protein expressed in Mycobacteria smegmatis mc2 155.Conclusion The Mycobacterium shuttle expression plasmid pJHSP70,which was based on shuttle plasmid pJEM11,has been constructed successfully." @default.
- W2360415618 created "2016-06-24" @default.
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- W2360415618 date "2008-01-01" @default.
- W2360415618 modified "2023-09-28" @default.
- W2360415618 title "Construction and identification of Mycobacterium shuttle expression plasmid pJHSP70" @default.
- W2360415618 hasPublicationYear "2008" @default.
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