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- W2360417265 abstract "Adenovirus vectors,expressing siRNAs in cell are the alternative delivery methods,which could block the target gene expression for longer time than that siRNAs transfected directly.To evaluate the transfect efficiency of replication deficiency adenovirus vectors and establish the RNA interference(RNAi) technical platform in chick embryo fibroblast(CEF),the transfect efficiency of GFP recombined adenovirus vector(adv-GFP) mediated in CEF cells is evaluated in this study.The results show that GFP gene expression is observed in CEF cells,with the delivery of adv-GFP from 0.1～1000 MOI.GFP positive cells are seen 16h post infection.GFP presents both in caryon and cytoplasm.The numbers of GFP positive cells and brightness of fluorescence reacted peak value during 24～36h,decayed subsequently and disappeared in about 180h.The highest transfect efficiency,22.3%,is achieved with 1 MOI adv-GFP delivery.The results in this experiment show that adv-GFP is safe and feasible to transfect CEF cells with no cytopathy and cell activity lost.GFP gene expression mediated by adv-GFP in CEF is repressed observably,with 85% interference efficiency,by synthesized anti-GFP siRNA transfected with cationic liposome reagent.We find that the RNAi mechanism is present in CEF cells like in other type cell.RNAi technique platform in CEF cells is established successfully in this study." @default.
- W2360417265 created "2016-06-24" @default.
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- W2360417265 date "2008-01-01" @default.
- W2360417265 modified "2023-09-24" @default.
- W2360417265 title "The expression and RNAi of GFP gene adenovirus vector mediated in chick embryo fibroblast cells" @default.
- W2360417265 hasPublicationYear "2008" @default.
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