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- W2360418518 abstract "Objective:To observe the effects of mycobacterium tuberculosis components (mannose-capped lipoarabinomannan,ManLAM) on the maturation of dendritic cells and downstream immunity.Methods:The peripheral blood mononuclear cells were obtained from healthy individuals,and then were cultured in medium with GM-CSF and IL-4.Five days later the medium was changed and cytokine was added to.On the sixth day,the cells were divided into three groups.Meanwhile ManLAM and LPS were added to stimulate the DCs according to the experimental design.Seven days later,the cells were collected and the level of DC-SIGN,HLA-DR,CD86 and CD83 were detected by flow cytometry.The levels of IL-12 and IL-10 in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA).Proliferative induction of DCs to CD4+T lymphocytes was measured by mixed lymphocyte reaction(MLR).The naive CD4+CD45RA+ T cells and allogeneic DCs were co-cultured for 48 hours.Subsequently,the supernatant IFN-γ level in each group was measured by ELISA.Results:(1)The expression of CD86 and CD83 on DCs in ManLAM group was lower than those of the mature group (P0.05).(2)In ManLAM group,the IL-10 level in the supernatant was higher and the IL-12 level was lower than those in mature group (P0.05).(3)The potency of proliferative induction of DCs to CD4+T cells and the activation of DCs from naive CD4+ CD45RA+T cells to Th1 cells in ManLAM group were lower than those in mature group(P0.05).Conclusion:The maturation of DCs could be restrained by ManLAM,so are the proliferation of T cells induced by DCs too and the activation of nave CD4+ CD45RA+T cells into Th1 cells." @default.
- W2360418518 created "2016-06-24" @default.
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- W2360418518 date "2010-01-01" @default.
- W2360418518 modified "2023-09-23" @default.
- W2360418518 title "Effects of ManLAM on the maturation of dendritic cells and downstream immunity" @default.
- W2360418518 hasPublicationYear "2010" @default.
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