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- W2360428237 abstract "Objective To construct an in vitro three-dimensional angiogenic model infected by over expression of adenovirus,thus to set up a platform for studying some specific gene in angiogenesis.Methods Blank adenovirus and cdc42 over expression adenovirus were prepared.Blank adenovirus was used to infect primary human microvascular endothelial cell(HMVEC),then the in vitro three-dimensional angiogenic model was constructed with the infected HMVECs,as well as the control model with noninfected HMVECs.The effect of infection on the growth of the angiogenic model was observed.cdc42 over expression adenovirus was used to infect primary HMVEC to construct the in vitro three-dimensional angiogenic model with the infected HMVECs,simultaneously blank adenovirus was used to infect the control model with HMVECs.The time the over expression lasted in the model developed was measured by reverse transcription PCR.Results There was a significant difference between average length of sprouts(ALS) of blank adenovirus infected models and ALS of noninfected models at the time points 24,48,72h(P0.01) repectively.There was no significant difference between ALS of the two models at the time points of 96 and 120h(P0.01).The over expression of cdc42 was maintained in the model at least for a week.Conclusions Adenovirus has been successfully used as a vector to induce over expression of some specific gene in the in vitro three-dimensional angiogenic model.A platform for studying accurate role of some specific gene in angiogenesis has been established." @default.
- W2360428237 created "2016-06-24" @default.
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- W2360428237 date "2007-01-01" @default.
- W2360428237 modified "2023-09-23" @default.
- W2360428237 title "Construction of an adenovirus infected in vitro three-dimensional angiogenic model" @default.
- W2360428237 hasPublicationYear "2007" @default.
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