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- W2360434564 abstract "Using orthogonal design and single factor trial,four factors including Mg2+,dNTPs,primers as well as Taq polymerase were screened and optimized in order to establish SSR reaction system in Kernelled Apricot(Armeniaca vulgaris Lam.).The results showed that 1.5 mmol/L Mg2+,1.5 U Taq polymerase,0.2 mmol/L dNTPs,0.25 μmol/L primers were the optimal conditions in the SSR-PCR reaction of 20 μL volume.The optimal annealing temperature was about 5℃ less than the least Tm.Through the optimized reaction system,SSR fragments of 24 Kernelled Apricot samples could be obtained,and the polymorphism between different samples was abundantly detected by 2% agarose gels,which showed this system was suitable and stable." @default.
- W2360434564 created "2016-06-24" @default.
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- W2360434564 date "2009-01-01" @default.
- W2360434564 modified "2023-09-24" @default.
- W2360434564 title "Optimization and Establishment of SSR Analysis Method in Kernelled Apricot" @default.
- W2360434564 hasPublicationYear "2009" @default.
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