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- W2360488918 abstract "The fertilized eggs of mirror carp (Cyprinus carpio var. specularis.) were Received July 7, 1997, revision received March 12, 1998 The project has been supported by the 863' project and the Fund of State Key Larboratory of Freshwater Ecology and Biotechnology. This study was completed in the State Key Laboratory, Institute of Hydrobiology, Thse Chinese Academy of Sciences To whom the correspondence should be addressed microinjected with an all-fish recombinant gene pCAsIGFc, a salmon insulin-like growth factor-I cDNA(sIGFc) capped with a carp actin gene promoter and the upstream region in pUC118. The polymerase chain reaction (PCR) assay showed that the transgene was detectable in all recipient embryos and in 80% of the 5 months old founders' caudal fin. For the caudal fin-transgenic founders, however, the transgene was only found in part of other tissues. The reverse transcription-polymerase chain reaction (RT-PCR) assay showed that the transgene expression occurred in 2/3 of gene-transferred embryos and in part of tissues of the 5 months old transgenic founders. The results indicate that existence and expression of the CAsIGFc in tissues of the transgenic horror carp is heterogeneous." @default.
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- W2360488918 date "1999-01-01" @default.
- W2360488918 modified "2023-09-28" @default.
- W2360488918 title "The Expression of Salmon Insulin-like Growth Factor I cDNA in Mirror Carp" @default.
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