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- W2360492009 abstract "Objective To reverse P-glycoprotein-mediated muhiple drug resistance using RNA interference(RNAi)in cultured rat astrocytes.Methods Astroeytes overexpressing P-glyeoprotein induced by coriaria lactone were transfected with the short-hairpin RNA expression vector-p-SIREN shuttle designed to target Mdrl mRNA.The mRNA level of Mdrl gene was evaluated by real-time PCR;the P-glycoprotein was examined by immunocytochemistry and image analysis,meanwhile the rhodamine efflux was assessed by flow cytometry.Results The astrocyte model overexpressing P-glycoprotein were established and successfully transfected with the short-hairpin RNA expression vector-p-SIREN shuttle.The mRNA level of Mdrl gene was knocked down by 67.70%(P0.01).The expression of P-glycoprotein in the experimental group was significantly lower than that in negative control,and the rhodamine efflux ratio of experimental group(23.08%)was remarkably lower than that of negative control(78.35%,P0.01).Conclusions RNAi can knock down the expression of Mdrl in cultured rat astrocytes induced by coriaria laetone and then significantly reverse drug resistance.This methodologic strategy may pave a new way of further research of refractory epilepsy." @default.
- W2360492009 created "2016-06-24" @default.
- W2360492009 creator A5081645624 @default.
- W2360492009 date "2007-01-01" @default.
- W2360492009 modified "2023-09-23" @default.
- W2360492009 title "Suppression of Mdr1 gene using RNA interference in cultured rat astrocytes induced by coriaria lactone" @default.
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