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- W2360500243 abstract "[Objective] To construct tetracycline (Tet) -controlled inducible vector pTRE-Tight-Ang-1, and then determinate expression character of Ang-1 under the regulation of Dox in cadiocyte. [Method] Ang-1 gene was amplified by nested PCR from the fresh lung tissue cDNA and the sequence was compared with Genebank data, and then sub-cloned into the pTRE-Tight plasmid after sequence analysis. PTRE- Tight-Ang-1 and pTet-on-Advanced were cotransfected with Lipofectamine 2000 TM to neonatal rat cadiocyte, expression of Ang-1 was assayed by Western blot after giving different concentration of Dox. [Results] The full-length of Ang-1 was obtained by nested PCR and identified by sequencing. Western blot showed that expression of Ang-1 could be induced by Dox in cadiocyte and shown in dose-dependent manner.[Conclusion] Tet-on inducible recombinant vector of pTRE-Tight-Ang-1 was successfully constructed, and its expression can be induced by DOX. This work has laid foundations for further study on IHD (ischemic heart disease)." @default.
- W2360500243 created "2016-06-24" @default.
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- W2360500243 date "2008-01-01" @default.
- W2360500243 modified "2023-09-24" @default.
- W2360500243 title "Construction and identification of human Angiopoietin-1 eukaryotic expression vector regulated by Tet-on Advanced system" @default.
- W2360500243 hasPublicationYear "2008" @default.
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