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- W2360533718 abstract "AIM: To study the method of culturing human primordial germ cells (PGC) and its differentiation in vitro and in vivo. METHODS: Primordial germ cells (PGC) collected from the genital ridge of 5～9 week (post fertilization) human embryos were cultured on feeder layer cells of primary mouse embryo fibroblast (PMEF) and human embryo fibroblast (HEF) with or without conditioned medium (rat myocardial cells) instead of leukemia inhibitory factor (LIF) which is a conventional way to culture primdial germ cells (PGC) and embryo stem cells (ES). RESULTS: These cultures were maintained on feeder layers for 4 passages, and under appropriate conditions embryo bodies (EB) and multiple differentiated cells phenotypes formed in monolayer culture and tumor (teratoma) formed in nude mouse. The characteristics of clone were similar to those of ES: big caryon, close cell arrangement and obscure boundary. CONCLUSION: Time and methods of separating genital ridge and PGC clone are of primary importance in the culture of PGC. PMEF and HEF have no significant difference as feeder layer in cutltuting PGC. Conditioned medium (rat myocardial cells) can effectively inhibit PGC differentiation.." @default.
- W2360533718 created "2016-06-24" @default.
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- W2360533718 date "2003-01-01" @default.
- W2360533718 modified "2023-09-23" @default.
- W2360533718 title "Derivation of pluripotential stem cells from cultured human primordial germ cells" @default.
- W2360533718 hasPublicationYear "2003" @default.
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