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- W2360539759 abstract "Objective To clone cinnamate 4-hydroxylase(C4H) gene from Isatis indigotica and to analyze its bioinformatics and expression. Methods The full-length c DNA of C4 H was 1 674 bp(Gen Bank accession No. GU014562) long with an open reading frame(ORF) of 1 530 bp encoding a polypeptide of 509 amino acid residues. The comparison of C4 H genomic DNA sequences and C4 H c DNA sequence revealed that the C4 H genomic DNA contained two introns. Southern-blotting analysis indicated that C4 H was a multiple copy gene. C4 H expression could be detected in all tissues at different expression levels, with the strongest expression in the roots. Further expression analysis revealed that the signaling components of defense/stress pathways, such as ultraviolet-B radiation(UV-B), methyl jasmonate(Me JA), abscisic acid(ABA), and Gibberellins(GA3) could up-regulate the C4 H transcript levels compared with the control. Conclusion We have first extracted the full length c DNA of C4 H gene from I. indigotica, and its structural and bioinformatic analyses are carried out, which will help us to further illuminate this pathway. The research also provides a possibility to study the antiviral active constiuents in I. indigotica by plant secondary metabolic engineering." @default.
- W2360539759 created "2016-06-24" @default.
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- W2360539759 date "2015-01-01" @default.
- W2360539759 modified "2023-09-28" @default.
- W2360539759 title "Molecular cloning and expression analysis of cinnamic acid 4-hydroxylase gene from Isatis indigotica" @default.
- W2360539759 hasPublicationYear "2015" @default.
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