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- W2360556117 abstract "ICE1 encodes a MYC-like b HLH transcriptional activator,which could specially bind to active domain of CBF3 promoter and induce transcriptal expression of cold-responsive genes downstream of CBF3,play important roles in stress response. A 1 247 bp sequence of Pns ICE1 promoter was cloned from DNA of Populus simonii × P. nigra by PCR amplification method. By sequence analysis,it consisted of a typical core promoter region of eukaryotic,beside the basic elements: TATA-box and CAAT-box,and there were some hormone responsive elements and multiple stress-induced elements of ABRE,DOFCOREZM,MYBCORE,MYCCONSENSUSATHSE and W-box. Pns ICE1 promoter may associated with adversity stress and play important roles in response to various stresses in P. simonii × P. nigra. To verify the biology function,recombinant vector was designated as p BI121-ICEPro through replacing Ca MV35 S promoter in p BI121 by cloned Pns ICE1 promoter fragment. In the vector,gus reporter gene was driven by Pns ICE1 promoter. GUS histochemical assay in Arabidopsis thaliana after transient infection showed that gus expressed in both flower and root." @default.
- W2360556117 created "2016-06-24" @default.
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- W2360556117 date "2015-01-01" @default.
- W2360556117 modified "2023-09-23" @default.
- W2360556117 title "Cloning and Transient Expression Analysis of Promoter of PnsICE1 from Populus simonii×P. nigra" @default.
- W2360556117 hasPublicationYear "2015" @default.
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