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- W2360561662 abstract "OBJECTIVE To clone and express the active v-Src protein, provide a PTK source for screening anti-tumor agents via Src inhibitors. METHODS The pGEX-KT vector was employed in the cloning of v-src gene. The expressed protein was purified, using GSH Sepharose 4B, and its characteristics were identified by Western blot analysis and ELISA technique. RESULTS The amount of GST-Src protein expressed from BL21 (DE3)pLysS culture exceeded 30% in relation to the coproteins. In 1 L medium culture, approximately 2 mg GST-Src fusion proteins with 85% purity and 13.5 mg GST-Src inclusion bodies with 98% purity were obtained. More-over, their tyrosine kinase ac-tivies reached about 0.62 nmol·min-1·mg-1 and 1.13 nmol·min-1·mg-1, respectively. CONCLUSION The GST-Src fusion protein expressed in this study was of high product, easy to be purified and prefer-able in the kinase activity." @default.
- W2360561662 created "2016-06-24" @default.
- W2360561662 creator A5061298101 @default.
- W2360561662 date "2005-01-01" @default.
- W2360561662 modified "2023-09-28" @default.
- W2360561662 title "High efficient expression,purification and activity analysis of v-Src from Escherichia coli expression system" @default.
- W2360561662 hasPublicationYear "2005" @default.
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