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- W2360566129 abstract "10-23 DNAzyme is one of antisense oligo-deoxynucleotides (asODNs) which actively split mRNA molecules. The split activity was used for verifying the binding validity of asODNs to different targeting sites on mRNA. For 4 sites on the mRNA of green fluorescence protein (GFP), four asODNs and four 10-23 DNAzymes were designed in parallel. The control asODN had 2 mutation bases from asODN of the best site 2, and control 10-23 DNAzyme contained 2 mutation bases in the binding arms of the 10-23 DNAzyme to site 2.In vitro cleavage of GFP transcript by DNAzyme and asODNs dependent RNase H found that they have the same order in efficacy. After intracellular transfection of HeLa cells with 4 kinds of DNAzymes and 4 asODN,respectively,immuno_fluorescence microscopy examination indicated similar order in knockdown effects by reducing fluorescence intensity with most significant attenuation occurring for A4 and D4.The GFP knock down effectiveness by 10-23 DNAzyme were identical to those of respective asODNs as revealed by flow cytometry.These results suggested that 10-23 DNAzymes may be a novel tool for verifying the validity of target sites on gene mRNA." @default.
- W2360566129 created "2016-06-24" @default.
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- W2360566129 date "2005-01-01" @default.
- W2360566129 modified "2023-09-23" @default.
- W2360566129 title "10-23 DNAzyme as a Novel Tool for Verifying the Validity of mRNA Target Sites" @default.
- W2360566129 hasPublicationYear "2005" @default.
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