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- W2360590897 abstract "In order to study the genetic diversity of Disanthus cercidifolius var.longipes,an endangered plant in China,improved CTAB method used to extract genomic DNA and this DNA as a template for ISSR-PCR reaction conditions were optimized.The optimal ISSR-PCR system for D.cercidifolius var.longipes was established for the first time,that is,20 μL of the reaction system,with 2 μL 10 × buffer,0.20 mmol·L-1 dNTP,0.25 μmol·L-1concentration of primers,2.0 mmol·L-1 Mg2+,1.0 U TaqDNA polymerase,about 20 ng DNA,14.1 μL double-distilled water.The optimal amplified procedure was as follows: after a pre-denaturing of 5 min at 94 ℃,followed by 36 cycles which were performed with denaturing of 30 s at 94 ℃,annealing of 1min due to denaturing temperature 48~54 ℃of different primer 45 s,extension 90 s of 1.5 min at 72 ℃,a final extension step of 7 min at 72 ℃and hold at 4 ℃.This paper could settle favorable basis for studying the genetic diversity of D.cercidifolius var.longipes using ISSR molecular marker techniques." @default.
- W2360590897 created "2016-06-24" @default.
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- W2360590897 date "2010-01-01" @default.
- W2360590897 modified "2023-09-26" @default.
- W2360590897 title "Optimization of ISSR-PCR reaction system for endangered plant Disanthus cercidifolius var. longipes" @default.
- W2360590897 hasPublicationYear "2010" @default.
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