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- W2360601480 abstract "A rapid detection of hepatitis E virus(HEV) gene chip with nano-amplification technique was developed.The whole frame can be described with sandwich hybridization of double probes coupled with silver stain enhancement.To detect Hepatitis E Virus,the virus RNA was extracted from the patient serum,then followed by RT-PCR.5′-end-NH2 modified oligonucleotides were designed to fabricate a DNA microarry in order to discriminate the target biotinylated DNA amplified by RT-PCR,the whole reaction system with partial dTTP was replaced by bio-dUTP,the signals generated with this method results from the precipitation of silver onto nanogold particles bound to streptavidin.The signals can be seen with naked eyes,which reduces the cost of detection.A total of 86 sera were tested by FQ-PCR and our homemade gene-chip.52 HEV positive samples tested by FQ-PCR all exhibit positively when tested by our rapid assay;These results suggested that chip-based detection method was consistent with a reference Fluorescence Quantitative PCR assay." @default.
- W2360601480 created "2016-06-24" @default.
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- W2360601480 date "2007-01-01" @default.
- W2360601480 modified "2023-09-23" @default.
- W2360601480 title "Visual Gene Detection Chip for HEV Based on Bio-Signal with Nano-Amplification Technology" @default.
- W2360601480 hasPublicationYear "2007" @default.
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