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- W2360614466 abstract "Objective To establish a sequence specific primers polymerase chain reaction (SSP-PCR) to detect single nucleotide polymorphisms of multidrug resistance gene 1 (MDR1) C3435T and G2677T. Methods SSP-PCR reacting condition was optimized, and the PCR results were compared with DNA sequencing. Blood samples of 158 healthy Hans were collected, and the polymorphisms in C3435T and G2677T of MDR1 gene were detected. Results The results of optimized SSP-PCR were coincidence with DNA sequencing. The MDR1 C3435T genotype frequencies were 37.34% (n = 59) for C / C, 11.39% (n = 18) for T / T, and 51.27% (n = 81) for C / T. The MDR1 G2677T allele frequencies were 44.30% for G, 39.24% for T, and 16.46% for A. The distribution of polymorphisms of C3435T and G2677T in Hans were statistically different from Caucasian (P 0.05). Conclusion The SSP-PCR method provides a fast and reliable approach for determining MDR1 polymorphism." @default.
- W2360614466 created "2016-06-24" @default.
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- W2360614466 date "2009-01-01" @default.
- W2360614466 modified "2023-09-27" @default.
- W2360614466 title "Detection of single nucleotide polymorphisms of multidrug resistance gene 1 C3435T and G2677T" @default.
- W2360614466 hasPublicationYear "2009" @default.
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