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- W2360616786 abstract "Objective To explore immunity response induced by DC-EBV-LMP2 with intramuscle immunity.Methods Bone marrow cells were separated from BALB/C mice,and cultured in RPMI1640 medium supplemented with 10% fetal calf serum and recombinant murine cytokines in vitro.Murine dendritic cells(mDCs) were harvested,and infected by recombinant adenovirus including latent membrane proteinⅡ(LMP2) gene(rAd-LMP2) of EpsteinBarr virus(EBV)at a dose of 100 multiplicity of infection(MOI).DC-EBV-LMP2 was induced and matured at 48 hours later.The LMP2 expression in mDCs was detected by immunoenzyme.BALB/C mice were immunized with 2×105DC-EBV-LMP2 per mouse by intramuscle immunity at 0,2,4 weeks.LMP2 specific cytotoxic lymphocytes(CTLs) and T cell subset in BALB/c mice were detected at 5th week and 8th week with enzyme-linked immunosorbent spot(ELISPOT) and flow cytometry,respectively.Results LMP2 specific CTLs in BALB/c mice were detected both at 5th week and 8th week after intramuscle immunity with DC-EBV-LMP2.LMP2 specific CTL in BALB/c mice is more powerful at 5th week(308±167 per 106cells,negative control 18± 6 per 106cells) than at 8th(196±81 per 106cells,negative control 7±2 per 106cells).The ratio of CD8+T to CD3+T subset is higher at DC-EBV-LMP2 group than injected PBS and DC groups at 5th week,but lower than in PBS and DC groups at 8th week.The antibody to LMP2 was detected in mice after DCEBV-LMP2 vaccinated.Conclusion LMP2 specific CTLs can be induced by the means of intramuscle immunity DC-EBV-LMP2 in BALB/c mice." @default.
- W2360616786 created "2016-06-24" @default.
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- W2360616786 date "2012-01-01" @default.
- W2360616786 modified "2023-09-23" @default.
- W2360616786 title "The Study of Immunity Response Induced by DC-EBV-LMP2 with Intramuscle Injection" @default.
- W2360616786 hasPublicationYear "2012" @default.
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