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- W2360632016 abstract "Poly(A)-binding protein(PABP) is one class of the highly conserved proteins found in many eukaryotes.PAPB was shown to play a role in regulation of mRNA stability and translation efficiency by binding the Poly(A) tail of mRNA and forming a translation initiation complex.Here we reported the cloning of BmPABP gene from the silkworm(Bombyx mori) by RT-PCR method.BmPABP contains four exons and a 1 812 bp open reading frame(ORF) which encodes a putative protein of 603 amino acids.BmPABP possesses four typical RNA recognition motifs(RRMs) and one conserved C-terminal domain.Analysis of microarray data showed that BmPABP expressed in a high level in different tissues of day 3 of 5th instar larvae and at developmental stages during day 4 of 5th instar larvae to adult moth.To determine the function of BmPABP,a transient expression vector pSLA4-BmPABP containing BmPABP gene under the control of B.mori Actin4(A4) promoter was constructed.The pSLA4-BmPABP was mixed with pSLA4-LUC containing luciferase reporter gene in a mole ratio of 1 ∶1 and 2 ∶1 respectively and then co-transfected into Sf9 cells.Luciferase activity was measured 72 hours after transfection.The results showed that co-transfection of pSLA4-BmPABP significantly increased luciferase activity by 11.9-fold and 7.5-fold,respectively.These studies show the ability of BmPABP to stimulate the expression of target genes,which exhibit a promising future application of BmPABP to enhance translational level of foreign genes and production of recombinant proteins in transgenic silkworms." @default.
- W2360632016 created "2016-06-24" @default.
- W2360632016 creator A5013698242 @default.
- W2360632016 date "2011-01-01" @default.
- W2360632016 modified "2023-10-01" @default.
- W2360632016 title "Cloning,Sequence and Functional Analysis of Poly(A)-binding Protein Gene BmPABP in the Silkworm,Bombyx mori" @default.
- W2360632016 hasPublicationYear "2011" @default.
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