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- W2360643561 abstract "Objective To study the change of cytokines related to innate immunity of human vaginal epithelial cells in candidiasis,and to investigate pathogenesis of vulvovaginal candidiasis further.Methods Human normal vaginal epithelial cells were cultured in vitro by tissue culture and keratinocyto serum-free medium(K-SFM).Passaging epithelial cells were cocultured with candida albicans medium in separate wells for 0,3,6,12,24,48 h. Epithelial cells cultured alone in separate wells were selected as control group.For examination of cytokines and chemokines,at each time point,the coculture supernatant and the control culture were collected.ELISA was applied to detect the levels of HBD-1,HBD-2 and IL-10 protein expression in the vaginal epithelial cells.Results Compared with the control group,the vaginal epithelial cells secreted more HBD-1,HBD-2and IL-10 at the time of 48 h in the model group,(P0.05). Conclusions Human vaginal epithelial cells have congenital anti-Candida albicans role and can secrete HBD-1,HBD-2,SP-A and IL-10 in face of Candida albicans infection." @default.
- W2360643561 created "2016-06-24" @default.
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- W2360643561 date "2009-01-01" @default.
- W2360643561 modified "2023-09-25" @default.
- W2360643561 title "The Effects of Innate Immunity of Human Vaginal Epithelial Cells on Vulvovaginal Candidiasis" @default.
- W2360643561 hasPublicationYear "2009" @default.
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