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- W2360761907 abstract "Cellar glutathione peroxidase (cGPX) is an important antixidase in animals. In this article RT-PCR method is developed for the semiquantitative analysis of cGPX mRNA. 18s rRNA is used as internal standard. Total RNA is extracted from pig muscle tissue. The mRNA is reverse-transcripted and the cDNA fragments of cGPX and 18s rRNA gene are amplified by RT-PCR using the gene specific primers. The key conditions in PCR process, the optimal cycle number and Mg 2+ concentration, are determined by studying the linear range of PCR amplification products. The integrated optical density(IOD) is showed by scanning the eletrophoresis bands of PCR products with VDS system. The relative concentration of cGPX mRNA can be calculated by the IOD ratio of cGPX and 18S rRNA." @default.
- W2360761907 created "2016-06-24" @default.
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- W2360761907 date "2005-01-01" @default.
- W2360761907 modified "2023-09-27" @default.
- W2360761907 title "RT-PCR method for the semiquantitative analysis of cGPX mRNA level in pig muscle" @default.
- W2360761907 hasPublicationYear "2005" @default.
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