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- W2360772511 abstract "The lactase gene from Pyrococcus furiosus was studied in this experiment.The cloned gene was expressed in Escherichia coli and the properties of lactase were determinated.celB gene was amplified by PCR and subsequently cloned,then ligated to the pET-30a(+) vector.The recombinant plasmid pET-celB was transformed into E.coli BL21.The positive transformants were induced at 28℃ for four hours by IPTG.SDS-PAGE analysis and lactase activity assay showed that celB gene was over-expressed in E.coli.The molecular weight of the recombinant expressed lactase CELB was about 58 kDa.CELB is a thermostable lactase.The optimum temperature was 105℃and better heat stability was achieved between 90℃ to 110℃.The optimum pH value was 5.0 and better pH stability was gained from pH 5.0 to 10.0.It is concluded that the lactase has a very weak dependence on metal ions." @default.
- W2360772511 created "2016-06-24" @default.
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- W2360772511 date "2008-01-01" @default.
- W2360772511 modified "2023-09-24" @default.
- W2360772511 title "Expression of β-Galactosidase from Pyrococcus furiosus in E. coli and Analysis of Lactase Properties" @default.
- W2360772511 hasPublicationYear "2008" @default.
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