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- W2360772534 abstract "The caprine osteoblasts of fetus were obtained from long bone periosteum, and cultured in DMEM at 37℃ with different bone membrane explants, explants with complete enzyme digestion and incomplete digestion,respectively. The results indicated that adhesion time, cell numbers and confluence time were 4h, 24h, 4h, respectively; few, few, large number of cells; and 21d, 19d,10d, respectively. The merging osteoblasts were found from passage culture on day 7, and there were apparent mineralized nodules on day 14. Von kossa and Alizarin red staining showed positive with jacinth and black respectively. Triangular, polyangular cells and obvious cell process were observed. While the cells have large nuclei and 1-3 nucleoli by Giemsa and H.E. staining. There were black deposits through Alkaline phosphatase(ALP) staining. Rough cell process was observed, which join and overlap cells under scanning electron microscope. Compared with primary culture, no bio-characteristic changes were observed from the 15th (filial) cells. It was concluded that incomplete digestion has less harmful to osteoblasts and is a kind of feasible method for bone tissue engineer, and the osteoblasts have good ability of attachment, fairly short culture period." @default.
- W2360772534 created "2016-06-24" @default.
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- W2360772534 date "2005-01-01" @default.
- W2360772534 modified "2023-09-24" @default.
- W2360772534 title "Cultivation and identification of caprine osteoblast in vitro" @default.
- W2360772534 hasPublicationYear "2005" @default.
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