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- W2360781382 abstract "Objective To observe the absorption of quercetin by the primary cultured rat hepatocytes and the protective effects of quercetin on those hepatocytes against oxidative stress.Methods We determined the absorption of quercetin on the primary cultured rat hepatocytes by high performance liquid chromatography(HPLC).H2O2 was added in the culture medium to induce oxidative stress on hepatocytes.In other experiments,rat hepatocytes were pretreated by quercetin,and then added H2O2 to observe the activity of lactate dehydrogenase(LDH)and total antioxidative capacity(T-AOC)in the culture medium,the content of malondialdehyde(MDA)in the cell homogenate,and the rate of cell apoptosis.Results The rat hepatocytes absorbed quercetin,reaching the peak at 24 hours.H2O2 caused injury of the rat hepatocytes.The contents of released LDH,the T-AOC,and the content of MDA were higher in the H2O2 group than those in the controls,so as the rate of apoptosis.Quercetin decreased the amount of LDH released from the hepatocytes and the content of MDA in the cell homogenate,increased the T-AOC in the medium,and lowered the rate of cell apoptosis.Conclusion The primary cultured rat hepatocytes can absorb quercetin,and quercetin has some protective effects on hepatocytes against oxidative stress." @default.
- W2360781382 created "2016-06-24" @default.
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- W2360781382 date "2007-01-01" @default.
- W2360781382 modified "2023-09-25" @default.
- W2360781382 title "Protective Effects of Quercetin on Primary Cultured Rat Hepatocytes against Oxidative Stress" @default.
- W2360781382 hasPublicationYear "2007" @default.
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