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- W2360797235 abstract "To enhance the expression level of Goose parvovirus(GPV) VP2 through baculovirus expression system(BES),the codon usage of GPV VP2 gene was optimized according to the codon bias of insect cells.The recombinant GPV with codon-optimized VP2 gene was then used to infect Sf9 insect cells.The expressed products were characterized in SDS-PAGE,Western blot and indirect immunofluorescence assay(IFA).A protein of molecular mass of 65 kDa reacted specifically with GPV VP2 antiserum.Immunofluorescent staining showed that codon-optimized VP2 was expressed in Sf9 cells.The virus-like particles(VLPs) of about 30 nm in diameter were observed under electron microscopy.The expression level of codon-optimized VP2 was significantly higher than the wild type.These findings indicated that VLPs derived from codon-optimized GPV VP2 products expressed in Sf9 insect cells yielded were useful for development of diagnostic reagents and vaccines." @default.
- W2360797235 created "2016-06-24" @default.
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- W2360797235 date "2012-01-01" @default.
- W2360797235 modified "2023-09-24" @default.
- W2360797235 title "CHARACTERIZATION OF VIRUS-LIKE PARTICLES DERIVED FROM CODON-OPTIMIZED GOOSE PARVOVIRUS VP2 GENE PRODUCTS EXPRESSED IN INSECT CELLS" @default.
- W2360797235 hasPublicationYear "2012" @default.
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