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- W2360806404 abstract "OBJECTIVE To study the influencing factors of induction and cultivation of Glycyrrhiza uralensis hairy root and its flavonoids production.METHODS By using genetic transformation of Agrobactierium rhizogenes and liquid cultivation technique,the induction and cultivation of Glycyrrhiza uralensis hairy root and its flavonoids production were investigated.RESULTS Different inducement frequencies of hairy roots were found among different A.rhizogene strains.A4 exhibited the strongest infection ability with explants.The percentage of rooted cotyledonary node explants after infection was more than 96%.The inducement frequency from cotyledonary nodes was the highest among different explants.Hairy root could be initiated from cotyledonary node explants 3-4 d after inoculation with the strain of A.rhizogene A4.The largest growth rate during hairy root cultivation in vitro was shown with inoculum size of 0.3 g and volume of 500 mL.The wet weight of hairy root after liquid culture was 41 times higher than before.The hairy root could produce medicinal secondary metabolites,flavonoids,and the highest amount of flavonoids in the hairy root reached a level of 2.042% dry weight and was 4.3 times of those in the untransformed root.The highest amount of flavonoids in 100 mL liquid culture was 1.36 mg.CONCLUSION The cultivation conditions of Glycyrrhiza uralensis hairy root and the content of total flavonoids have been systematically established.The results presented here have made it possible for large scale cultivation of G.uralensis hairy root and production of flavonoids." @default.
- W2360806404 created "2016-06-24" @default.
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- W2360806404 date "2011-01-01" @default.
- W2360806404 modified "2023-09-26" @default.
- W2360806404 title "Study on Induction and in Vitro Cultivation of Glycyrrhiza uralensis Hairy Root and Its Flavonoids Production" @default.
- W2360806404 hasPublicationYear "2011" @default.
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